Fermentas pcr purification kit manual

GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 min and the isolated DNA is ready to use in all common downstream applications including ligation, restriction digestion, PCR, sequencing and labeling. Fig. 1. Recovery dependence on DNA fragment Missing: fermentas. The kit was tested in the purification of PCR products according to the protocol described in the manual. The quality of the purified DNA was evaluated spectrophotometrically, by agarose gel electrophoresis, digestion with Thermo Scientific FastDigest restriction enzymes and automated fluorescent sequencing. Quality authorized by: Jurgita ZilinskieneFile Size: KB. QIAquick PCR Purification Kit Protocol using a microcentrifuge This protocol is designed to purify single- or double-stranded DNA fragments from PCR and other enzymatic reactions (see page 8). For cleanup of other enzymatic reactions, follow the protocol as described for PCR samples or use the MinElute Reaction Cleanup www.doorway.ru Size: 33KB.

QIAquick PCR Purification Kit Protocol using a microcentrifuge This protocol is designed to purify single- or double-stranded DNA fragments from PCR and other enzymatic reactions (see page 8). For cleanup of other enzymatic reactions, follow the protocol as described for PCR samples or use the MinElute Reaction Cleanup Kit. High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis. Highlights. Efficient-2 to 10 µg of genomic DNA from mL of blood. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each GeneJET spin column can recover up to 20 µg of plasmid DNA. The kit can be successefully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and.

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica based membrane technology in the form of a convenient spin column eliminating the need for tedious resin manipulations or toxic phenol chloroform extractions It effectively removes primers dNTPs unincorporated labeled nucleotides enzymes and salts from PCR and other reaction mixtures The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to Each GeneJET purification. The QIAquick PCR Purification Kit and the QIAquick PCR Gel Cleanup Kit (cat. nos. , , and ) can be stored at room temperature (15–25°C) for up to 12 months if not otherwise stated on label. The GeneJET PCR Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 25 bp to 20kb with recovery rates up to %. Each GeneJET purification column has a total binding capacity of up to 25μg of.